The Laboratory Guide to Gnotobiotic Research is a comprehensive guide that conveys the importance of gnotobiotics to overall scientific discovery. It also focuses on how the addition of a gnotobiotic facility to an individual institution can provide an impactful contribution to the overall success of its research program and open tremendous opportunities for research funding. The book introduces the reader to the fundamental process of establishing a new gnotobiotic facility and includes important considerations such as: securing institutional support, determining funding sources, and establishing the critical design team to consider structural requirements.